Hyperlipidemia is one of the major causes of disability in developing countries. It is the one of the major risk factor of coronary heart diseases. The present study was designed to investigate the effect of the pod extract of a medicinal plant Acacia nilotica (AN) on fructose-induced hyperlipidemia in rats. The plant extract and commercial lipid lowering drug atorvastatin were administered in fructose-induced hyperlipidemic rats (FIHRs) at a dose of 100mg/kg and 200mg/kg per day.Biomedical parameters were studied includingSerum Triglyceride, Serum Total Cholesterol, Serum LDL, Serum HDL, VLDL, in control, treated and diabetic rats. The result of the experiment suggest that The AN extract showed a significant decrease in lipid profile, i.e it shows antihyperlipidemic effect in fructose induced hyperlipidemic rats.