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A high-content imaging method for monitoring C. elegans embryonic development

Authors: Wang, Shaohe; Ochoa, Stacy; Khaliullin, Renat; Gerson-Gurwitz, Adina; Hendel, Jeffrey; Zhao, Zhiling; Biggs, Ronald; +4 Authors

A high-content imaging method for monitoring C. elegans embryonic development

Abstract

This repository includes the following files: embryoCropUI_WINDOWS- PC version of executable GUI embryoCropUI_MacOS- Mac version of executable GUI (requires MacOS X10.11) Instructions- Instructions for installation and running GUI (embryoCropUI) and FIJI script (OpenAndCombine.ijm) Test_files- Contains two test files: a multi-tif format file and a folder with a tif image series, which can be used to test the GUI on your system (details for testing can be found in the instructions) OpenAndCombine_embs.ijm- FIJI script that enables assembly of maximum intensity projections for multiple embryos within one viewer. Note this works with our file structure and will need to be modified to accommodate your file structure (see instructions).

source code for the embryoCropUI executable can be found at Github: https://github.com/renatkh/embryo_crop.git

Keywords

C. elegans, embryo, crop

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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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