The use of radiofrequency serves the purpose of improving dermal aging through new collagen fibres formation, making the skin firmer. The objective of this study is to investigate the effects of fractional radiofrequency on the dermal tissue of the palpebral region in women. The research was carried out with 10 volunteers of mean age 53.5 ± 4.95 years, who presented upper palpebral skin sagging. The patients underwent four sessions, one every fifteen days. The procedure was performed with the volunteers in dorsal decubitus, using the fractional radiofrequency equipment with 20 mJ of energy, and electrodes of 8 or 25-needle tips during ten minutes. The assessments required for treatment were performed at all sessions and fifteen days after the last session for results comparison. In the histological analysis, only one volunteer was submitted to blepharoplasty, in which two samples of the skin flap of the upper palpebral region were removed. The results were positive, both in clinical and histological analyses, even though smaller semiablative doses were used. The results showed a significant improvement in the whole region, showing greater presence of collagen, as well as of inflammatory cells, confirming that the treatment using fractional radiofrequency for skin flaccidity has its effectiveness at clinical and histological levels.