N-Hydroxy N- (2 methyl) phenyl N’ (2fluoro) phenyl benzamidine hydrochloride is newly synthesised reagent.Mo (VI) is reduced to Mo (V) with ascorbic acid in hydrochloric acid solution and then complexed with thiocyanate. The orange red complex is extracted with benzene solution of N-Hydroxy N (2 - methyl) phenyl N’ (2 -fluoro) phenyl benzamidine hydrochloride and thiocyanate. The coloured mixed chelate absorbs at 470 nm, with molar absorptivity 3790/ mole-1lit. Beer’s law is obeyed in the range is 5.0-20ppm of molybdenum.The optimum acidity range is 2.0 to 3.4 MHcl. Most of the common ions including Al (II), Cr (III), Fe (III), Fe (II), Ni (II), Zr (IV), V(V), Ti (IV), Zn (II) etc do interfere. The method has been used for the determination of molybdenum in soil samples.