Tripospermumlimneticum sp. nov.encountered on dead decaying submerged leaves of Eucalyptus sp. and Polygonumglabrum found in Purna River (Dang district), Gujarat, India is described and illustrated here. This species is distinguished by its freshwater habitat, branched conidia arms (mostly four) are horizontal and downwardly directed, perpendicular to the stalk cell, obtuse and hyaline at the tip and here proposed as a new species.