pyClamp is a feature-complete graphical user interface written in Python to demonstrate the flexibility of the novel dynamic clamp implementation: The current user interface focuses on data consistency (between the interface and the dynamic clamp system) and data transparency (with the option of monitoring any values live). Furthermore, a lab journal will keep track of new experiments and record all relevant settings with corresponding timestamps. Cross-reference: dyClamp is a further development of the dynamic_clamp sketch with a focus on a robust serial communication between the Teensy and its controlling host computer. This software was partially developed at Laboratory of Catherine Proenza, Department of Physiology & Biophysics, University of Colorado, Anschutz Medical Campus (NIH R01-HL088427).