abstract: Data were collected in the summer 2017 in a second order stream with the objective to draw an hydromorphological map thru the entire stream reach (1150 meter). To do to this, the stream was characterized from its habitat types. This dataset contains the location of each habitat type presents along the stream. Six habitat types were used to characterize the stream hydromorphology (based on the Bisson classification). Waterfall base, cascade, riffle, run, glide and pool. Method: The study was conducted in Gregoire Stream, a second-order stream in southern Quebec, Canada (46°05'40''N, 71°25'58''W). The area has a rain-snow regime characterized by peak water flows in spring and low flows in summer. We delineated a reach of 1150 m in length characterized by a flat low-flow section at the upstream end (stream distance = 1150 m) and a series of waterfalls at the downstream end (stream distance = 0 m), near to the confluence of Gregoire Stream and the Bagot River. The reach was crossed by a road which separated two zones differing in slope and riparian vegetation; a downstream zone having steeper slope and forest cover and an upstream zone having shallower slope and mostly shrub cover. Hydromorphologic habitat features were categorized in habitat units using the classification system of Bisson et al. (1982). Six habitat types were found: pool, glide, run, riffle, cascade, and waterfall base. We measured the length of each habitat unit along the reach to the nearest 0.5 m, yielding a measure of the length of each unit. The entire stream reach was visually mapping after its habitat types at a precision of 0.5 meter. The characterization was performed using the classification system of Bisson et al. (1982), distinguishing six habitat types: pool, glide, run, riffle, cascade, and waterfall base. Results gave a serie of habitat unit along the stream reach with sometimes two different lateral habitats units.