This contains the R script and data files needed to reproduce all analysis and graphs in the manuscript: Guo, H., & York, L. M. (2019). Maize with fewer nodal roots allocates mass to more lateral and deep roots that improve nitrogen uptake and shoot growth. Journal of Experimental Botany. academic.oup.com/jxb/advance-article/doi/10.1093/jxb/erz258/5506708 The ZIP file includes a .R text file to be opened in RStudio and 17 CSV data files. The user should set the working directory in R to be the same as the location of the .R file and the accompanying CSV files. All packages needed are at the top of the script and need installed before running. Once these dependencies have made, running the R script will reproduce all analysis and graphs from the manuscript.