Traumatic Brain Injury (TBI) is a significant civilian health problem. TBI triggers complex changes on the central nervous system. NSE and GFAP may provide drug target identification. We aim in this study to investigate the possible effects of pyrimidine derivatives in the recovery of TBI. Materials and methods: Research was conducted on 80 male animal rats, with body weights varying from 200-220g with experimental brain injuries (?weight-drop method?). The concentration of NSE and GFAP and the level of cerebral edema were estimated after the injury in animals that were treated with pyrimidine derivative (50 mg/kg) and choline alfoscerate (50 mg/kg). Results: The level of biomarkers in group of negative control was elevated. After the treatment the experimental animals with pyrimidine derivative GLY and choline alfoscerate the concentration of NSE and GFAP decreased as well as cerebral edema. Conclusion: Administration of GLY one hour after injury at a dose 50 mg/kg minimizes cerebral edema and the damage of neurons.