The aim of this study is to isolate and identify Species of blood-contaminated bacteria in Blood bank refrigerators which they are out of service for several reasons can be diagnosis and identification via Five samples (Swabs) which they are prepared from inside of leftedrefrigerator at the stores of the private nursing hospital at the city of Baghdad, samples are cultured on blood agar media, after 24 hours, results showed three species of bacteria, these bacteria are diagnosed via biochemical and enzyme-based tests according to Bergey\'s Manual 2005, which they are Staphylococcus aureus, Bacillus cereus,andEnterococcus faecalis. These species are diagnosed because of their ability to resist environmental conditions for a long time. There are other species of bacteria are not being diagnosed because of inability to resist dryness or changing of temperature degrees or sterilization.