Introduction: Analysis of urine protein plays an important role in the evaluation of patients with suffering from renal disease. Analysis of 24h urine gathering was for quite a while the strategy for decision for measuring proteinuria however is never again suggested on the grounds of burden and imprecision because of human blunder in accumulation. Aims and objectives: The basic aim of the study is to analyze spot urine protein vs creatinine ratio as a predictor of 24hr urinary protein excretion in nephrotic syndrome. Material and methods: This cross sectional study was conducted at Mayo Hospital, Lahore during October 2018 to December 2018. The data was collected from 150 patients of both genders. Early morning urine examples were gathered as spot urine and estimated for urinary protein, creatinine and protein to creatinine ratios. That day, 24-hour urines were gathered from 8 am to 8 am. Blood tests were drawn for serum creatinine (mg/dL). Results: The data were collected from 150 patients from which 90 were male and 60 were female. The mean age was 25.6 ± 11.7 years. The mean value of serum creatinine was 85.8 ± 19.6 (umol/L) and urine creatinine was 6.2 ± 1.6 (mmol/L) in selected patients. The Pakistani children and grown-up patients who were discharging in excess of 150 mg urinary protein for every day; uncovered protein: creatinine record and ratio 141 and 0.18 individually in irregular urine tests. The concentration of protein in the urine is influenced by urine volume just as protein discharge rate. Conclusion: It is concluded that Urine prot vs Urine creatinine ratios in random urine sample are best indicators of 24h urinary total protein excretion in patients with and without renal insufficiency. Key words: Urinary, Protein, Creatinine, Ratio, Analysis.