Background: Blood transfusion carries the major risk of transmitting infections such as hepatitis, HIV, syphilis, and malaria, which needs a proper and serious monitoring to provide safe blood for transfusion. Aim: To determine the seroprevalence of Transfusion Transmitted Infections (TTIs) and to evaluate trends in TTIs among blood donors Materials and method: The present study was carried out in Department of Transfusion medicine, Meenakshi Medical College Hospital, Kanchipuram. Study period was from January 2018 to December 2018. A total number of 1588 donors were screened for HIV, HBV, HCV, syphilis and malaria by ELISA method. Results: Out of 1588 donors, 95.84 % were males 4.16 % were females. 1.008 % were reactive for transfusion transmitted infection. HBV 0.94%, and HCV 0.06%were the major infections in blood donors. None were positive for HIV, syphilis and malaria Conclusion:Seropositivity of HBV, HCV, HIV, syphilis and malaria among the healthy donors indicates the risk or chance of acquisition of these infections during blood transfusion. Implementation of donor selection criteria should be strict and proper to minimize transfusion transmitted infections and provide safer blood and blood products and also higher generation kits and newer strategies are to be adopted.