Pseudarthriaviscidaa member of Fabaceae family possess great medicinal attributes.The present study dealt with antimicrobial ability of chloroform extract P. viscidaand its associated major fungal endophyte and their phytochemical analysis via GCMS. Pseudarthriaviscida yielded two endophytesColletotrichumgloeosporioides (Pv-1) and C. lindemuthianum (Pv-2)and isolation frequency resulted C. gloeosporioides (Pv-1) to be major one. Chloroform extracts at different concentrations (25, 50, 75 and 100μl) ofP. viscidaleaves, mycelial mat (?g/ml)and culture filtrate (v/v) of major endophyte C. gloeosporioides(Pv-1) were tested for their antimicrobial activity against two bacterial species, Escherichia coli and Staphylococcus aureus and two fungal species, Fusariumoxysporum and Cladosporiumcladosporioidesrespectively. The extracts were found to show inhibitory activity against all the studied pathogens. GC-MS analysis of both plant and fungal endophyte resulted in the presence of 16 compounds from P. viscidaplant extracts, 16 from 16 from Pv-1 C.F. and 20 from Pv-1 M.E. In case of P. viscidaand its endophyte Pv-1, 2,4-Ditert-butylphenol, E-14-Hexadecenal, E-15-Heptadecenal, 1-Hexadecene, Cyclotetracosane, Octadecyltrifluoroacetate were similarbut their area percentage varied. Majority of the compounds from extracts were identified to possess therapeutic properties and further chemical evaluation is needed for its successful application.