Personality has become a focus point in organizational research over the past couple of decades, management skills development and derailment, and mainly employee selection. A better understanding of the personality traits of members of the profession is also required; to deliver a complete pictures of the way in which pharmacists should attempt to revolutionize pharmacy practice. A descriptive cross-sectional study design was to assess personality traits among pharmacists working in different fields in Pakistan. Sample size was calculated to be 382 pharmacists to achieve 95% confidence level with 5% margin of error. A pre-validated data collection tool Big Five Inventory questionnaire was self-administered to the respondents. After data collection, data was cleaned, coded and analyzed using SPSS version 21. Results showed that out of 382 respondents, females were found more extrovert than males with a mean score (25.1, ±3.89). Agreeableness was found low in all studied fields of pharmacy. Neuroticism was high in industrial pharmacists with mean score (26.5, ±4.3) and hospital pharmacists with mean score (27, ±4.1). Consciousness was found high in pharmacists working in private sector with mean score (29.1 ±4.6). Openness was low in pharmacist working in both private and government sectors. The present study concluded that although, neuroticism a negative trait was found high among pharmacists in twin cities but it was encouraging to notice that pharmacists also possessed positive traits including extraversion, consciousness and openness in their personality. Moreover, industrial pharmacists, hospital pharmacists and especially pharmacists working in government sectors possessed highest neuroticism.