The preset study was framed out to evaluate the phytochemicals and the antibacterial activity of the fractions separated from crude extracts of the Terminaliachebula, Momordicacharantia, Dregeavolubilis.Methods: Phytochemical analysis was carried out using standard such as Mayer?s test, Wagner?s test, Dragendorff test, Hager?s Test, Alkaline copper test, Lead acetate test, Ferric chloride (FeCl3) test to identify the presence of secondary metabolites such as alkaloids, Flavonoids, Tannins in the fractions separated from the above selected plants. Results: The test carried out for screening of phytochemicals was given positive. According to results we noticed the presence of different types of secondary metabolites such as Alkaloids, Phenols, Flavanoids, Tanins, Saponins, Carbohydrates, Amino acids in the leaf fractions. The fractions inhibited the growth of bacterial strains used in the study and exhibited antibacterial activity. The results were compared with standard reference drug ciprofloxacin at 10 mg/mL. Based on our results, it is noted that, the chloroform, ethanol and aqueous fractions are more active than n-hexane, toluene fractions. The least MIC value (12 and 16?g/ml) was recorded with ethanol and aqueous fractions of Momordicacharantia respectively. Conclusion: fractions of separated from theDregeavolubilis, Terminaliachebula, Momordicacharantiamedicinal plants (leaves) had significant phtochemicals which are responsible for antimicrobial activity.