Soil bacterium capable of utilizing organophosphate based pesticides as sole carbon source was isolated by selective enrichment on mineral medium containing chlorpyrifos and malathion individually. A total eleven bacterial isolates were isolated from five different agriculture soils. The pesticide tolerance test revealed that MGPD-02 was high potential isolate against chlorpyrifos and malation, biochemical characteristics revealed that MGPD-02 was gram negative bacilli citrate, nitrate reductase, MR and VP positive bacteria. Growth studies showed that MGPD-02 utilized chlorpyrifos to grow in Luria-Bertani broth containing different concentrations of chlorpyrifos at 50 -500 ppm. However, the optimum concentration that supported bacterial growth over 24 h was found to be 50 -200 ppm. Further concentrations were proved to be decreasing their bacterial growth and become completely lethal at 500 ppm for both chlorpyrifos and malathion.