India has a strong history of traditional herbal system of treatment of various health ailments. In spite of substantial advances in medicinal plant research and rapid advances in modern medicine, there was an increasing problem of liver disorders. The aim of the current study was to perform preliminary phytochemical screening, HPTLC finger printing profile and quantitative analysis of macronutrients, minerals including protein pattern by SDS-PAGE analysis for the various extracts of Melia azedarach lin leaves and seeds of Piper longum respectively. The study reveals that preliminary phytochemical screening of Melia azedarach extract; Piper longum extract shows the presence of constituents like alkaloids, carbohydrates, phytosterols, tannins, phenol, flavonoids, glycosides, terpenes, lignin. HPTLC fingerprinting profile and quantitative analysis for both plants of various extracts showed the presence of active components will be responsible for therapeutic activities like antioxidant, anti-emetic, anti-oxidant activity and hepato-protective activity. In future, further studies are needed with these plants to assess, isolate and characterize their pharmacological potentials with their medicinal values.