According to the International Diabetes Federation, the challenges of early stage diagnosis and treatment effectiveness monitoring in diabetes is currently one of the highest priorities in modern healthcare. In this experimental study, the potential of combined measurements of skin fluorescence and blood perfusion by the laser Doppler flowmetry method in diagnostics of low limb diabetes complications was evaluated. With the use of Monte Carlo probabilistic modelling, the diagnostic volume and depth of the diagnosis were evaluated. The experimental study involved 76 patients with type 2 diabetes mellitus. These patients were divided into two groups depending on the degree of complications. The control group consisted of 48 healthy volunteers. The local thermal stimulation was selected as a stimulus on the blood microcirculation system. Experimental studies have shown that diabetic patients have elevated values of normalised fluorescence amplitudes, as well as a lower perfusion response to local heating. In the group of people with diabetes with trophic ulcers, these parameters also significantly differ from the control and diabetes only groups. Thus, the intensity of skin fluorescence and level of tissue blood perfusion can act as markers for various degrees of complications from the beginning of diabetes to the formation of trophic ulcers.

The work was supported by grant of the President of the Russian Federation for state support of young Russian scientists № MK-7168.2016.8. Authors acknowledge the support of the Academy of Finland (grants: 296408 and 290596). EAZ also acknowledged for personal support H2020 MCSA-funded project No. 703145. The authors would like to acknowledge the patients and volunteers. Special thanks are extended to doctors Alimicheva E.A., Masalygina G.I. and Muradyan V.F. of the "Orel Regional Clinical Hospital" for providing useful advice and help.