Urinary tract infections are the most recorded infections in the microbiology laboratory. The enterobacteria are the most commonly isolated bacteria in these types of infections. The present work aims to study the frequency of isolated strains of Enterobacteriaceaecytobacteriological urinalysis from the microbiology laboratory of Mohamed V Tangier hospital and determine their resistance patterns to antibiotics. This is a retrospective study, which took place over a period of 24 months (January 2013 to December 2014) on 2716 urine samples, 410 examinations met the criteria for urinary tract infection including 325 (11.9%) Enterobacteriaceae strains were isolated. The identification was done by API 20 E galleries, sex ratio female / male was 1.69, Isolated bacterial species are dominated by Escherichia coli with 259 (83%), at its enterobacterial strains resistance to amoxicillin was 88.6%, and 68.3% in the protected amoxicillin, resistance to trimethoprim + sulfam?thoxazoles was equal to 33.2%, resistance to fluoroquinolones (ciprofloxacin) was 20.3%, aminoglycosides (Gentamycin) is 14.15%, fosfomycin was 11.7%, while the sensitivity to 3rd generation cephalosporins (ceftazidime) was 7.7%. 14 cases of resistance to beta lactamase extended spectrum (ESBL) was recorded which represents 4.3%, while for cases (1.2%) of resistance to carbapenems were recorded among these isolated Enterobacteriaceae strains. This study appears to reinforce other studies that shed light on antibiotic resistance, which is an issue of growing concern which leads us to recommend a more focused use of antibiotics.