The aim of this study was to screen various extracts prepared from aerial part and root of Boenninghausenia albiflora for their metabolite profiling and in-vitro antioxidant activity. Arial part and root were subjected to quantitative screening test for various constituents which revealed the presence of sugar (6.5 and 8.4%), starch (7.0 and 10%), and tannins (37.5 and 36.96 μg tannic acid equivalent /mg dry extract), respectively. The order of total phenolic content was descended in following order: hydro-alcoholic extract of aerial part (HEA) methanolic extract of aerial part (MEA) hydro-alcoholic extract of root (HER)methanolic extract of root (MER). HEA showed an effective scavenging of DPPH radical (IC50, 195.9μg/mL) followed by MEA (IC50 243.8 μg/mL). While IC50 values for HER and MER were recorded by 370μg/mL and 600μg/mL, respectively. Based on IC50 values, the order of antioxidant activity based on β-carotene bleaching of different extracts was found to be MER MEA  HEA HER. Data revealed that act as an antioxidant agent due to its free radical scavenging activity. For achieving good separation of phenolic compounds using HPTLC, a mobile phase of toluene: ethyl acetate: formic acid (5:5:1) was used and data revealed the presence of caffeic, ferulic, gallic, and vanillic acids in roots and aerial part extracts of B. albiflora. The quantification of phenolic compounds and antioxidant activity in B. albiflora have not yet been reported, thus this information can be useful for proper standardization of herbal drug containing B. albiflora.