From ancient times, Medicinal plants were used for the treatment of various deadly diseases. Especially Asian countries like India, china etc., were used medicinal plants as a potential curing agent for various diseases and used in their different medicinal system named ayurvedic, siddha etc.,. Withania somnifera is an indigenous herb belonging to family Solanacae, commercially known as Ashwagandha. It is extensively used in most of the Indian herbal pharmaceuticals and nutraceuticals, It was spread all over the India and in some other Asian countries. It has phytochemicals named alkaloid, steroidal lactones, flavonoids, saponins and tannins; it has various pharmacological activities whereas in the present study we clearly investigate their antibacterial, antifungal and antioxidant activities in their leaves and root extracts. Among the various solvent used our research reveals that the Aqueous, chloroform and methanol extracts shows maximum inhibitory effect against the tested bacteria and fungi. In addition to that we proved that these extracts also have antioxidant activity. Furthermore we concluded that the aqueous leaf extract and methanolic root extract has virulent activities when compared to other.