Dyes are serious pollutants, causing environmental and health problems to human being and aquatic animals. Textile wastewater with dye contaminants presents a severe environmental peril because of persistent nature and allied toxicity along with bioaccumulation propensity. Therefore, treatment of dye effluents has become utmost important criterion prior discharged into the environment. In present study three different textile dyes namely, methylene blue, rhodamine B and reactive green 19, were selected for decolorization study using three types of water borne pathogens explicitly Bacillus subtilis, Staphylococcus aureus and Pseudomonas aeruginosa). Effect of major operational process parameters like, initial dye concentration, temperature and pH were investigated for decolorization of textile dyes and present study confirms the ability of Pseudomonas aeruginosa and Bacillus subtilis as potential decolorizing bacterial with efficiency of 97.28% for methylene blue and 98.73% for Reactive Green 19. Significantly high decolorization level and simplistic process conditions illustrate the potential for these bacterial strains to be used in the biological treatment of dyeing mill effluents.