Forward and reverse Sanger sequencing chromatograms (AB1 format) for fifteen bacterial isolates, from the near-full-length 16S rRNA gene amplified with the universal primers 27F and 1492R (~1,465 bp amplicon). These 15 read pairs (30 files) are the validation dataset for ChromaView, an open-source desktop application for Sanger chromatogram analysis. A mapping of study sample identifiers to file names and embedded ABI sample names is included (see README). Trimming, NCBI BLAST identification, and forward–reverse consensus assembly are described in the associated software (DOI: 10.5281/zenodo.20740237) and manuscript.