This dataset contains processed RNA-seq data supporting the transcriptomic analyses of gastrocnemius muscle from control, diabetic model, and static exercise-treated type 2 diabetic mice. The dataset includes sample metadata, FPKM expression matrices, gene annotation information, candidate gene expression tables, enrichment analysis outputs, original company-provided processed files, and README documentation. The RNA-seq analysis was used as an exploratory, hypothesis-generating component of the study and was followed by targeted molecular validation.