Hair loss, dandruff, dryness, and poor scalp health are common problems affecting both men and women. Continuous exposure to pollution, stress, nutritional deficiency, and chemical-based cosmetic products may damage hair follicles and reduce hair growth. Herbal formulations are increasingly preferred due to their natural origin, safety, and reduced side effects compared to synthetic preparations. The present study was aimed at the formulation and evaluation of a herbal hair growth serum containing horsetail millet extract as the major active ingredient. The serum was prepared using natural ingredients such as aloe vera gel, glycerin, jojoba oil, vitamin E oil, rose oil, and rose water. Horsetail millet extract was prepared using Soxhlet extraction method. The prepared serum was evaluated for organoleptic properties, pH, viscosity, spreadability, phytochemical screening, stability studies, and anti-inflammatory activity. The formulated serum showed satisfactory physicochemical characteristics with pH 5.4, viscosity 520 cps, and spreadability 6 cm. Phytochemical screening confirmed the presence of flavonoids and phenolic compounds. Stability studies showed no significant changes in color, odor, consistency, or phase separation under different storage conditions.