This position paper was developed by four Horizon Europe projects working at the biodiversity–health interface to support the European Commission in implementing the Strategy for European Life Sciences and to identify research gaps and priorities relevant to Horizon Europe for the next framework programme period 2028-2034.It draws on evidence generated by four Horizon Europe projects: BCOMING (Grant agreement ID: 1010594832), BEPREP (Grant agreement ID: 1010605683), RESTOREID (Grant agreement identifier: 1011349694) and ZOE (Grant agreement ID: 1011350945). The evidence base includes project deliverables, scientific articles and contributions, BCOMING, BEPREP, RESTOREID8 and ZOE policy briefs, as well as a cross-project questionnaire completed by partners across all four projects. The questionnaire was developed to identify shared scientific findings, implementation challenges, research gaps, infrastructure priorities, and policy needs related to biodiversity, One Health, and pandemic preparedness, and to support the development of joint recommendations for Horizon Europe 2028–2034.