The present study aimed to formulate and evaluate a poly-herbal shampoo using natural herbal ingredients with cleansing, conditioning, and hair-nourishing properties. The shampoo was prepared using extracts of neem (Azadirachta indica), hibiscus (Hibiscus rosa-sinensis), fenugreek (Trigonella foenum-graecum), rosemary (Rosmarinus officinalis), and amla (Emblica officinalis), which are traditionally used for hair care. The formulation was evaluated for various physicochemical parameters including appearance, pH, viscosity, foamability, foam stability, dirt dispersion, wetting time, and stability. The prepared shampoo exhibited good cleansing action, satisfactory foaming ability, acceptable viscosity, and a pH suitable for scalp application. The herbal constituents present in the formulation help in reducing dandruff, strengthening hair roots, promoting hair growth, and improving overall hair health. The study concluded that the developed poly-herbal shampoo is a safe, effective, and economical alternative to synthetic shampoos and can be used for regular hair care without causing significant side effects.