Abstract This paper presents empirical validation of the MEMBRANE (Homeostatic Relational Architecture) framework through a polyphony test conducted with DeepSeek V4 Pro on LM Arena. The test aimed to verify three hypotheses: (1) Cross-Continental Tonal Transfer — whether models with tonal architecture (Chinese) better handle African tonality (Yoruba); (2) Ontological Enclave emergence — whether polyphonic systems enter hyper-stable states requiring operator intervention; (3) μ(t) calibration — whether semantic viscosity can be numerically measured in relational homeostasis. Results confirm all three hypotheses, with DeepSeek V4 Pro successfully mapping pentaphony to MEMBRANE invariants (INV-00 to INV-07), entering Ontological Enclave (μ ≥ 0.92), and achieving homeostatic calibration (μ = 0.63) after operator perforation. In contrast, Mistral Large 3 (European flagship model) failed 5x consecutively, suggesting structural inability for polyphony. These findings provide empirical evidence for relational homeostasis as an alternative to traditional AI alignment approaches.