The increasing public demand for interdisciplinary educational programs in the botanical gardens is much wider and more diverse than required by the curricula of different educational institutions. In this work we present the results of a study conducted as a part of specially organized classes for the students of Pharmaceutical College in Peter the Great Botanical Garden (St. Petersburg, Russia) in 2022. The classes were aimed at developing environmental competences of pharmacy students in the fields of botany, plant ecology, applied ecology and human ecology, in order to improve environmental literacy of the future professionals. The research of this kind is viable and necessary, both for comparing the results, and for the general development of the environmental education (EE). Preliminary research stage, which included diagnostic assessment of the students’ subjective attitude towards nature level using Naturaphyl questionnaire, showed that average component values in students of the two groups generally correspond to the average adolescent values. The individual components of the students’ attitude towards nature are dominated by the perceptual/affective scale (P/A), proving the aesthetic nature of environmental attitudes. The diagnostic data was used for the methodological work and development of study units using case method. The suggested course of classes in the Botanical Garden shows the effectiveness of environmental education for the pharmacy students.