This research which evaluated the impact of produced water on Oryclotagus cuniculus, assesses its effect on kidney metabolites, after 30 days of exposure to sublethal concentrations of toxicant in a randomized experiment. A small portion of the kidney from experimental animals were collected by dissection, crushed in a ceramic mortar, harmonized with deionized water, and centrifuged for 15 minutes at 3000 rpm after the exposure. The supernatant was collected in sample bottles for metabolites assay. Kidney metabolites shows significant disruption where urea levels significantly increased in all treatment group peaking at 6.60 mg/L (7.77±3.78 µmol/L) compared to control that recorded (3.36±0.65 µmol/L) at 0.00mg/L. This also impacted on the creatinine which were also elevated across treatment groups, in particular at 13.30 mg/L and 20.00 mg/L compared to the control at 0.00mg/L.On the other hand, protein and bilirubin levels dropped respectively at 13.30 mg/L (2.79±0.74 g/L) compared to control that recorded 4.67±0.89g/L at 0.00mg/L and at 20.00 mg/L (16.96±18.65 µmol/L) compared to control that recorded 33.01±5.38 µmol/L at 0.00mg/L. These findings confirmed that produced water disrupts kidney metabolites exposed to rabbits, giving a scientific basis for advocating improved waste management practices and reinforcing environmental protection policies to mitigate the deleterious effects of oil industry effluents.