In this study, a novel, selective, sensitive, reliable, accurate, and robust RP-HPLC technique has been successfully developed and validated for simultaneous analysis of Amlodipine (AML) and Chlorthalidone (CHL) in their bulk powder and tablet formulation. Chromatographic analysis was carried out by HPLC system integrated with LC Solution software with Agilent Zorbax C18 column (250 × 4.6 mm, 5 µm). The maximum wavelength (λmax) at 242 nm. A mobile phase composed of 0.01 M buffer and acetonitrile (50:50v/v) was delivered with a flow rate of 1.0 mL/min. Under these conditions, Amlodipine (AML) and Chlorthalidone (CHL) exhibited retention times of about 3.0 min and 5.8 min, respectively. Validation of method was performed by assessing its linearity, accuracy, precision, and specificity. Linearity of the developed method with a coefficient of correlation for the standard curve is 0.9997 and 0.9996 for Amlodipine and Chlorthalidone respectively. The RSD for the precision of the method were found less than 2.0 %. The percentage recoveries of Amlodipine and Chlorthalidone were found to be 100.52 % and 100.04 % for respectively