This dataset consists of water column measurements collected from a trace metal CTD during the CLIVAR/GO‑SHIP repeat hydrography section P16S from 9 January to 16 February 2005, a meridional transect in the South Pacific from roughly 17°S to 71°S. Station metadata, hydrographic data, and cruise documentation are provided by the CLIVAR & Carbon Hydrographic Data Office (CCHDO) under expocode 33RR200501. A trace-metal clean rosette was used to collect seawater from the upper 1000 m of the water column and then subsampled in a clean van for salinity, nutrients, and dissolved trace metal samples. Filtered (<0.4 µm) samples were collected for shipboard analysis by flow injection methods for dissolved Fe, Al, and Mn concentrations and shore-based ICPMS analysis for Fe, Co, Ni, Cu, Zn, Cd, and Pb at Florida State University. The timestamp is in UTC. For a full description of sampling protocols and analytical methods see: C.I. Measures, J. Yuan, J.A. Resing, 1995. Determination of iron in seawater by flow injection analysis using in-line preconcentration and spectrophotometric detection, Limnol. Oceanogr. Methods, 50, 3-12. C.I. Measures, W.M. Landing, M.T. Brownand, C.S. Buck, 2008. A commercially available rosette system for trace metal-clean sampling, Limnol. Oceanogr. Methods, 6, 384-394. A. Milne, W. Landing, M. Bizimis, P. Morton, 2010. Determination of Mn, Fe, Co, Ni, Cu, Zn, Cd and Pb in seawater using high resolution magnetic sector inductively coupled mass spectrometry (HR-ICP-MS), Anal. Chim. Acta, 665, 200–207. J.A. Resing and M.J. Mottl, 1992. Determination of manganese in seawater using flow injection analysis with on-line pre-concentration and spectrophotometric detection. Analyt. Chem., 64, 682-687. J.A. Resing, C.I. Measures, 1994. Fluorometric determination of Al in seawater by flow injection analysis with in-line preconcentration, Anal. Chem., 66, 4105-4111. We thank the captain and crew of R/V Roger Revelle and chief scientists Bernadette Sloyan and James Swift.This research was funded by NSF grants OCE-0223378 to William M. Landing (Florida State University) and OCE-0649584 to Christopher I. Measures (University of Hawaii).