This upload contains materials related to a cross-sectional study conducted among dry fish handlers in the southern coastal region of Bangladesh. The study assessed knowledge and safety practices regarding personal hygiene, environmental hygiene, and food safety using a structured questionnaire. Data were collected from 412 participants using multistage cluster random sampling. The study also examined socio-demographic and occupational determinants of hygiene knowledge and workplace practices. This material may be used for research and educational purposes in public health, food safety, and occupational health studies.