Azadirachta indica (Neem) is a well-known medicinal plant extensively used in traditional systems of medicine due to its broad spectrum of pharmacological properties. The present study aims to evaluate the in-vitro antidiabetic. anticancer. and anti-inflammatory activities of the ethanolic extract of Azadirachta indica leaves. The extract was prepared using Soxhlet extraction with ethanol as the solvent to obtain a wide range of bioactive compounds. Antidiabetic activity was assessed through a-amylase and a-glucosidase inhibition assays, which demonstrated significant dose-dependent inhibition of both enzymes, indicating potential to control postprandial hyperglycemia. Anticancer activity was evaluated by MIT assay on MCF-7 (breast cancer) and HeLa (cervical cancer) cell lines, where the extract exhibited concentration-dependent cytotoxicity with decreased cell viability. Anti-inflammatory activity was determined by protein denaturation inhibition assay. which showed significant inhibition of protein denaturation, suggesting anti-inflammatory potential. The findings of this study suggest that ethanolic extract of Azadirachta indica leaves possesses promising in-vitro antidiabetic. anticancer. and anti-inflammatory activities. which may be attributed to the presence of various phytoconstituents such as flavonoids, tannins. phenols. and terpenoids. This study supports the traditional use of neem and encourages further investigations for isolation and characterization of active compounds and in-vivo studies. Keywords: Azadirachta indica, Neem, Antidiabetic, Anticancer. Anti-inflammatory. In-vitro. Soxhlet extraction. a-amylase. a-glucosidase, MTV assay.