ABSTRACT:Trimethoprim and sulfamethoxazole popularly known to be as co-trimoxazole. It ispopular antibiotic used to treat various types of bacterial infections. Collagen is theprimary building block of our skin, muscles, tendons, ligaments and other connectivetissues which also has a role in wound healing. Both of these antibiotics and thecollagen together can help to prevent microbial growth as well as aids in woundhealing. Co-trimoxazole inhibits the bacterial growth by inhibiting folic acid synthesisand collagen provides structural integrity to the damaged tissue. Co-trimoxazole hasless absorption from external application which can be overcome by adding collagen,which will helpCo-trimoxazole to penetrate and prevent bacterial infection in the healing wounds.So, there is a scope for evaluation of topical formulations of Co-trimoxazole in different kind of wounds models.