Environmental factors, stress, and poor hair care practices are typical causes of dandruff, hair loss, and poor hair health. Because herbal formulations are safer, more effective, and have less adverse effects than synthetic medications, they have attracted a lot of interest. The creation and assessment of a polyherbal hair serum comprising neem (Azadirachta indica), hibiscus (Hibiscus rosa-sinensis), curry leaves (Murraya koenigii) extraction is the main goal of this work. The chosen herbal components are well-known for their positive benefits on the health of the scalp and hair. Curry leaves aid in strengthening hair follicles and lowering hair loss, aloe vera has moisturizing and conditioning effects, hibiscus encourages hair development and prevents premature greying, and neem has potent antimicrobial and anti-dandruff qualities. To create a stable formulation, the extracts were made using appropriate extraction techniques and added to a serum base. A number of physicochemical properties, such as pH, viscosity, spreadability, homogeneity, and stability, were assessed for the prepared serum. Its compatibility with the scalp and ability to promote hair growth were also evaluated. According to the findings, the formulation had good stability, acceptable physical properties, and the potential to improve hair texture, lessen dandruff, and stimulate hair growth. In conclusion, the created polyherbal hair serum has remarkable therapeutic potential for preserving healthy hair and scalp and can be regarded as a safe and efficient substitute for synthetic hair care products.[1]