This dataset includes relevant data and figures associated with the manuscript “Comparison of two laser wavelengths for LIBS bioimaging of plants grown in lunar regolith”. The Figures are in [png] formats and all LIBS data are in [libsdata] formats. Data files are labeled according to the corresponding figure in the manuscript. The dataset is organized in folders corresponding to figures. Only figures reporting experimental results are included in the dataset. Each folder reports processed images related to a specific Figure. Full context and detailed analysis of the provided raw and processed Data can be found in the manuscript.