This scientific article provides a comprehensive and in-depth analysis of the nervous and humoral regulation of cardiac activity from the perspective of modern physiology and pathophysiology. The heart is considered a central organ of the circulatory system with intrinsic automaticity; however, its function is continuously regulated by neural and humoral mechanisms that ensure adaptation to changing internal and external conditions. The study examines in detail the mechanisms of neural regulation, including the roles of the sympathetic and parasympathetic nervous systems. It is demonstrated that the sympathetic nervous system exerts a stimulatory effect on the heart by increasing heart rate, enhancing contractile force, and accelerating impulse conduction, whereas the parasympathetic nervous system produces inhibitory effects, reducing heart rate and overall cardiac activity. Particular attention is given to humoral regulation, focusing on the influence of hormones such as adrenaline, noradrenaline, and thyroxine, as well as the role of electrolytes (calcium, potassium, and sodium) in maintaining normal cardiac function. These factors are shown to play a crucial role in regulating myocardial excitability, conductivity, and contractility. Furthermore, the article highlights the interaction between neural and humoral regulatory mechanisms, which enables the cardiovascular system to adapt to physical activity, stress conditions, and resting states. The coordinated function of these systems is essential for maintaining homeostasis and ensuring the efficient performance of the cardiovascular system. In conclusion, the nervous and humoral regulation of cardiac activity is presented as a complex integrated system that plays a key role in sustaining life processes and facilitating physiological adaptation to environmental changes.