Abstract: Modern microscopy experiments generate terabytes of highly diverse data, creating major challenges for processing and analysis. In this talk, I will present our recent efforts to build open-source tools to address these challenges relying on the OME-Zarr format: ngio, a Python library to facilitate interactions with OME-Zarr files, and Fractal, a computational platform for scalable and modular bioimage analysis workflows on HPC infrastructures.