Abstract Fungal contamination is a major factor affecting the quality and storage life of oilseeds, particularly soybean, resulting in reduced germination, poor seed health, and economic losses. Although chemical pesticides are widely used for control, their long-term use raises concerns related to environmental safety and human health. Hence, the development of sustainable and eco-friendly alternatives has become essential. This study investigates the potential of Pseudomonas fluorescens as a biological seed coating agent to enhance the shelf life and quality of soybean seeds. The bacterium exhibits strong antagonistic activity against fungal pathogens through the production of antimicrobial compounds, siderophores, and lytic enzymes. It also promotes plant defense by inducing systemic resistance, thereby increasing the ability of plants to withstand infections. Seed treatment with Pseudomonas fluorescens helps in reducing fungal load, improving germination percentage, and enhancing seedling vigor. In addition, treated seeds show better resistance to storage fungi such as Aspergillus and Fusarium, leading to improved storability and quality. The findings of this study highlight the effectiveness of microbial seed coating as a sustainable approach for managing seed-borne pathogens and reducing post-harvest losses. This method offers a promising alternative to chemical treatments and supports the adoption of eco-friendly practices in agriculture