This study investigates the diversity, prevalence, host associations of helminth parasites in the gastrointestinal tract of freshwater fishes of Pravara River. The research was conducted over one year across multiple sites along the river to capture spatial and seasonal variations in parasitic infection. A total of 420 fish specimens representing 12 species were examined. Helminths belonging to trematodes, cestodes, and nematodes were recorded, with notable findings in carnivorous hosts (e.g., Clarias batrachus). Results demonstrate distinct helminth assemblages influenced by fish feeding behavior, habitat, and water quality.