Background: Work life Balance is an important phenomenon concerning a lot of working employees. Females form a major proportion of the works force in the healthcare industry and the policies should focus on their well-being for a stress free environment. Many of research has been carried out focusing on work life balance. However, there has been very few research concerning the work life balance of female healthcare professionals working in private and trust hospitals. Therefore, this study aims to assess the challenges and organizational support for the female healthcare professionals working in hospitals of Vadodara district (India).Methodology: The data collection tool is a structured close ended questionnaire where in the respondents were asked to choose their option pertaining to various parameters of work life balance. The data collected from 867 female healthcare professionals working in private and trust hospitals of Vadodara (India). The data collected were analyzed using SPSS version 23. The statistical test used was Kruskal-Wallis and Mann-Whitney test at 95% confidence level.Major Results: There were 304 female doctors and 563 nurses in the study. among 288 female healthcare professionals under 30, 270 had personal life expectations and workplace support, while 18 did not. Of the 161 aged 41-50, 160 had personal life expectations and support, and only 1 did not. A chi-square test confirmed a significant association between age and personal life expectations & workplace support (p < 0.05).Conclusions: Work life balance is a critical area of concern for employers within human resource area, therefore this study highlights a key issue that need to be address to enhance the satisfaction and well-being of female healthcare professionals.