Dataset for article: Save and Natural Innovation in Regenerative Medicine: Enhancing Collagen Scaffold Stability with Vitamin B2 (Riboflavin)-Mediated UV Crosslinking contains data including in the article. Table 1: List of prepared samples and overview of associated crosslinking and processing parameters Table 2: Comparison of tensile strength (UTC) and Young’s modulus (E) (mean ± standard deviation). Figure 1: Schematic illustration of riboflavin-mediated collagen crosslinking under ultraviolet irradiation. (png) Figure 2: CLSM analysis and visualization of porous collagen scaffolds: a) and c) crosslinked in 1 mM riboflavin and ethanol for 5 minutes; b) and d) crosslinked in 1 mM riboflavin and water for 5 minutes. Figure 3: SEM characterization of collagen scaffold morphology: (a) micrograph and pore size distribution histogram of the EDC/NHS-crosslinked scaffold used as a reference; (b) effect of riboflavin concentration (1, 10, 100, and 1000 µM; left to right) on scaffold morphology under UV-A and UV-C irradiation (15 min); (c) effect of UV irradiation time (5, 10, 15, and 30 min; left to right) on scaffold morphology under UV-A and UV-C irradiation (1000 µM riboflavin). All scale bars represent 200 µm. (jpg, excel table for pore size distribution) Figure 4: ATR-FTIR spectra of pure non-crosslinked collagen, EDC/NHS crosslinked scaffold, and riboflavin-UV crosslinked scaffolds, displaying the characteristic amide A, B, I, II, and III absorption bands. (unicode origin graph; to open it you need to have the origin available) Figure 5: Second derivative of FTIR spectra of the amide I band for scaffolds crosslinked at 1000 μM riboflavin concentration with varying UV irradiation times (5; 10; 15; and 30 min.) using a) longwave UV-A radiation, and b) shortwave UV-C radiation. Second derivative of FTIR spectra of the amide I band for scaffolds crosslinked at 15 minutes UV irradiation with varying riboflavin concentrations (1; 10; 100; 1000 μM) using c) longwave UV-A radiation and d) shortwave UV-C radiation. (unicode origin graph - to open it you need to have the origin available ; excel for processing of second derivative of FTIR spectra) Figure 6: Swelling properties of collagen scaffolds a) crosslinked at 1000 μM riboflavin concentration with varying UV irradiation times b) crosslinked at 15 minutes UV irradiation with varying riboflavin concentrations. (excel data) Figure 7: Degradation profiles of collagen scaffolds: a) and b) crosslinked at varying UV times; c) and d) crosslinked at varying riboflavin concentrations. (excel data) Figure 8: Viability of L929 fibroblast cells following exposure to extract media from scaffolds: a) crosslinked at 15 minutes UV irradiation with varying riboflavin concentrations (1; 10; 100; 1000 μM), b) crosslinked at 1000 μM riboflavin concentration with varying UV irradiation times (5; 10; 15; and 30 min.). The red line indicates the cytotoxicity limit. Significance levels are indicated as follows: p < 0.05 (*), p < 0.01 (**), and p < 0.0001 (****). Figure 9: (a) EDC/NHS; (b) SW 10 min; (c) SW 100 µM; (d) LW 10 min; and (e) LW 100 µM over a 24 h period. Wound closure increased over time, reaching near-complete closure after approximately 12–15 h. Due to the non-linear nature of wound closure kinetics, migration rates were quantified by linear regression restricted to the 6–12 h interval, where the response was approximately linear. The calculated slopes (%/h) were used to compare wound healing between groups. Statistical analysis (unpaired t-test vs. control) revealed significant differences in migration rates for all treatment groups (p < 0.05), with samples (b) and (e) showing highly significant reductions (p < 0.0001). (excel data + tiff figures) Supplementary Figure 1: Collagen scaffolds presenting fluorescence during the crosslinking process due to UV irradiation. (png) Supplementary Figure 2: Representative images of tensile test specimens prior to and following DMA measurements: a) SW100 µm before; b) SW100 µm after. (jpg) Supplementary Table 1: Statistical analysis results of swelling ratio for scaffolds crosslinked at varying UV irradiation times. (excel data) Supplementary Table 2: Statistical analysis results of swelling ratio for scaffolds crosslinked at varying RF concentrations. (excel data) Suplementary Table 3: Pairwise post-hoc comparisons with statistically significant differences in enzymatic degradation between samples studying the effect of UV irradiation time. Significance levels: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***). (excel data + pdf processing) Supplementary Table 4: Pairwise post-hoc comparisons with statistically significant differences in enzymatic degradation between samples studying the effect of riboflavin concentration. Significance levels: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***). (excel data + pdf processing)