The objective of the present research was to design and evaluate a novel herbal hydrogel containing Mimosa pudica extract for potential wound healing applications. Hydrogels are preferred topical drug delivery systems due to their ability to maintain hydration, promote healing, and enhance patient compliance. Mimosa pudica is a medicinal plant traditionally used for treating wounds, inflammation, and skin disorders. In this study, a hydrogel was formulated using a suitable polymeric base incorporated with a hydroalcoholic extract of Mimosa pudica. The prepared formulation was evaluated for physicochemical parameters including appearance, pH, viscosity, spreadability, swelling index, and drug content uniformity. In vitro diffusion studies were conducted to assess the release pattern of the extract, along with short-term stability studies. The results indicated that the developed hydrogel possessed acceptable physicochemical properties, uniform drug distribution, and sustained release behaviour. The study concludes that Mimosa pudica based hydrogel can serve as a promising herbal formulation for topical wound healing applications.