Background: Infection control is a cornerstone of patient safety and healthcare quality. Despite established guidelines, compliance among healthcare workers (HCWs) varies widely. Objective: To assess the knowledge, attitudes, and practices (KAP) related to infection control among HCWs in a tertiary care setting, nursing homes in Indian hospitals. Methods: A cross-sectional, multicentric hospital survey of 74 HCWs was conducted using an online structured questionnaire. Data were analyzed descriptively to identify gaps and barriers. Results: While 93% of participants were aware of infection control guidelines, only 55% consistently practiced hand hygiene before patient contact. Barriers included inadequate training (58%) and insufficient supplies (52%). Conclusion: Prevention of Infection in hospital and its control remains the biggest challenges still today. The findings of this study reflect high awareness contrasts with inconsistent practices. Targeted training and institutional support are essential to bridge this gap and reduce healthcare-associated infections (HAIs).