This Legacy Booklet summarises the key results of the EU project B-Cubed (Biodiversity Building Blocks for policy), which aimed to standardise access to biodiversity data.B-Cubed developed a service to create “species occurrence cubes”, which then served as the basis for models and an indicator of past, current and future biodiversity. To ensure reproducibility of its results, the project created exemplary workflows for modelling and indicator calculation, as well as a suite of guides, tutorials and hands-on training sessions. The application and usefulness of B-Cubed’s algorithms and software are demonstrated through the project’s four case studies, which consider a variety of environments, regions and challenges to maximise their relevance to stakeholders and policy-makers and to test the extremes of the tools.