Skin whiteners are kind of therapy methods that can be used for the treatment of hyperpigmentation. They inhibit the biosynthesis of melanin using tyrosinase inhibitors. Nowadays, lightening the skin tone is one of the most common procedure. Agaricus bisporus extract is used as a skin whiteining agent. The Agaricus bisporus bodies extraction was carried out by using soxhlet apparatus by using ethanol as solvent. The Percentage of yield was about 31%. Preliminary phytochemical test for extract was performed and showed positive results for tannins, phenol, terpenoids, saponin and alkaloids. Phytochemicals like phenol, tannins and terpenoids are for antityrosinase activity. A three formulation of skin whitening serum containing agaricus bisporus extract was prepared and evaluation parameters such as physical parameters, pH, viscosity, spredability and Antityrosinase activity was performed. Percentage of Tyrosinase inhibition of F3 formulation is highest as compare to the F1 and F2.