The bakery industry has emerged as one of the rapidly growing segments of the food processing sector in India, particularly in urban areas such as Pune City. The profitability of bakery enterprises depends largely on the adoption of effective business practices including pricing strategies, marketing activities, customer service quality, inventory management, and technology adoption. The present study examines the effect of business practices on profitability of bakery enterprises in Pune City. The study is based on primary data collected from 100 bakery enterprises through a structured questionnaire. Statistical tools such as percentage analysis, mean analysis, and regression analysis were used to examine the relationship between business practices and profitability indicators including sales growth, profit margin, and business expansion. The results of regression analysis indicate that business practices have a significant positive effect on profitability, with customer service and marketing practices emerging as the most influential determinants. Inventory management and pricing strategies also contribute significantly to cost efficiency and profit improvement, while technology adoption improves operational efficiency. The study concludes that adoption of modern business practices is essential for improving profitability and ensuring long-term sustainability of bakery enterprises.