ABSTRACTSeed size is one of the most important traits that significantly affectthe whole production, and seed color is greatly affected the price, preferenceand acceptability for consumers. Therefore, identifying and understandinggenes involved in the control of seed size and color are crucial andnecessary for mungbean improvement. However, many studies of specific-controlled genes of seed size and color have not been conducted. Theobjectives of this study were to investigate phenotypic variations in seedsize and color among mungbean accessions and to identify loci associatedwith seed size and color in cultivated mungbean accessions using genome-wide association study (GWAS). Phenotypic data were collected from seedas seed size and color. Principle component analysis (PCA) plots wereconstructed based on phenotypic data using 209 cultivated and 9 wildmungbean accessions from more than 23 countries. The cultivated and wildmungbean accessions in both seed size and color are clearly separated.Manhattan plots were constructed with a total 18,171 of SNP markersdeveloped using genotyping by sequencing (GBS) on 209 cultivatedmungbean accessions. Using GWAS to perform general line model (GLM).A single significant marker associated with red and green seed colorcomponents was identified on Chr04 at 3370425 bp with p-value of <0.005,while blue seed color component and seed sizes had no significant markerassociated with.