The production of bioethanol is an important process due to its wide applications in renewable energy. It also plays a significant role in waste management, as most agricultural by-products, including kitchen waste, can be converted into useful bioethanol. This process involves the use of enzymes for ethanol production, and enzyme activity can influence the yield and quality of the ethanol produced. Therefore, this study investigated the effect of different enzymes on bioethanol generation. The enzymes used were alpha-amylase, cellulase, and S. cerevisiae (baker's yeast). The slurry produced was transferred into a 1000 mL beaker, and the pH was measured and adjusted to 5.0. Alpha-amylase and cellulase were added simultaneously to facilitate hydrolysis, after which S. cerevisiae was introduced for fermentation. Ethanol production was carried out in a prefabricated bioreactor. The enzyme concentrations ranged from 0.1 g/L to 0.7 g/L, while pH, temperature, and substrate concentration were kept constant to evaluate the effect of enzyme concentration on bioethanol yield. The results showed that a continuous increase in enzyme concentration under optimal conditions led to a corresponding increase in bioethanol yield. The highest yield (80.4%) was obtained at the maximum enzyme concentration of 0.7 g/L. Additionally, the highest bioethanol concentration (15.4 g/L) was achieved at 35°C and a pH of 5.0.